High-grade brain malignancies
We built a glioblastoma atlas at single-cell resolution in adult patients that compiles different studies and created a curated reference map. Our resource provides an easy-to-use platform for cell annotation and hypothesis generation. Besides, we continue studying the cell heterogeneity and how in high-grade gliomas in children, the tumor is organized into distinct cellular communities to understand and unravel the role of the tumor microenvironment (TME) in cancer behavior and development. Finally, the local signaling environment between cancer cells and TME will be studied in intact tissues using spatial transcriptomics.
This project aims to establish a comprehensive single-cell reference map of human patient-derived organoids and matched primary issue to generate gene expression and open chromatin profiles from the same cell. With the reference map, we aim to provide a better insight between and understanding of health and disease.
Pediatric and adult AML
Acute Myeloid Leukemia (AML) is an aggressive hematopoietic malignancy with low overall survival; adult and pediatric AML patients suffer from high risk of relapse. To gain insight into the clonal heterogeneity during disease progression, we perform in depth (epi)genome analysis and single-cell RNA sequencing of bone marrow at diagnosis and relapse. In collaboration with the Zwaan and Heidenreich groups, we focus on diagnosis-relapse pairs in childhood AML. These experiments will advance our understanding of clonal evolution and treatment related changes in the composition.