Our website uses cookies. We use cookies to remember settings and to help provide you with the best experience we can. We also use cookies to continuously improve our website by compiling visitor statistics. Read more about cookies

High-throughput screening (HTS)

Our aim is to facilitate the accelerated identification and validation of improved treatment strategies and disease-associated genes and proteins by supporting the development, performance and analysis of high-throughput perturbation assays. The HTS facility is embedded in the Molenaar group at the Princess Máxima Center for pediatric oncology.
What we offer:

The highly flexible HTS platform enables the performance of a variety of high-throughput screens, using different perturbations (e.g. small molecules, antibodies or siRNAs) and read-out methods (e.g. absorbance-, luminescence- or fluorescence-based detection or flow cytometric analysis).
Assay development and validation – we can give support in the translation of research questions into assays suitable for high-throughput screening.
Carrying out the high-throughput screens
Data analysis support
Provide compound libraries:

  •   The Prestwick Chemical Library (Prestwick), containing 1280 marketed drugs with high chemical and pharmacological diversity.
  • The Anti-Neoplastic KIT (Sequoia), containing 165 (recently) approved drugs used in cancer treatment.
  • The Spectrum Collection (MicroSource Discovery Systems), containing a selection of 2320 drug components, natural products and bioactive molecules.
  • The Screen-Well® Epigenetics Library (Enzo), containing 43 drugs with defined activity against epigenetics-modifying enzymes.
  • The Epigenetics Screening Library (Cayman), containing 54 compounds that modulate the activity of epigenetic enzymes and histone-binders.
  • The Cell Cycle/DNA Damage Compound Library (MedChemExpress), containing 451 cell cycle/DNA damage related compounds.
Our equipment:

The HTS facility consists of a liquid handling robot for the accurate and reproducible distribution and (serial) dilution of single solutions into 96- and 384-well plates. Plates can be transferred to the following integrated devices for incubation, further sample processing or read-out:

  • Automated CO2 incubator – for incubation of standard and deep well plates
  • Microplate reader – for absorbance, luminescence and fluorescence detection
  • HyperCyt® Rapid Sampler combined with the BD AccuriTM C6 Flow Cytometer
  • Vspin integrated microplate centrifuge (Agilent) – a centrifuge for standard and deep well microplates
  • Multidrop Combi reagent dispenser (Thermo Fisher Scientific) – an automated pipetting system for solution dispensing into 96- and 384-well plates
  • D300e Digital Dispenser (Tecan) – a benchtop dispenser for the rapid and accurate delivery of picoliter to microliter volumes of small molecules and biomolecules in DMSO or aqueous solution into 12-, 24-, 48-, 96-, 384- and 1536-well plates. The D300 Digital Dispenser is useful for the estimation of dose-response curves, for combination studies, for the setup of PCR reactions and for the generation of enzyme profiles
  • Plate sealer and peeler – suitable for polypropylene and polystyrene plates

The liquid handling robot additionally contains on-deck modules for shaking, heating, cooling and washing.

Personnel

Facility manager
Emmy Dolman

Assay development and application
Bianca Koopmans

Assay development and application
Anke Essing

Interested?

Contact Emmy Dolman
Email: M.E.M.Dolman-2@prinsesmaximacenterum.nl