Our website uses cookies. We use cookies to remember settings and to help provide you with the best experience we can. We also use cookies to continuously improve our website by compiling visitor statistics. Read more about cookies

I agree
Support us
Support us

High-throughput screening (HTS)

Our aim is to facilitate the accelerated identification and validation of improved treatment strategies and disease-associated genes and proteins by supporting the development, performance and analysis of high-throughput perturbation assays. The HTS facility is embedded in the Molenaar group at the Princess Máxima Center for pediatric oncology.
What we offer:

The highly flexible HTS platform enables the performance of a variety of high-throughput screens, using different perturbations (e.g. small molecules, antibodies or siRNAs) and read-out methods (e.g. absorbance-, luminescence- or fluorescence-based detection).
Assay development and validation – we can give support in the translation of research questions into assays suitable for high-throughput screening.
Carrying out the high-throughput screens
Data analysis support
At the moment, we focus on drug screens with designed libraries specific for pediatric cancer.

Our equipment:

The HTS facility consists of a state-of-the-art robotic system, with as the main part the Beckman Coulter Biomek i7 Hybrid liquid handling workstation. It contains deck positions for tip boxes and assay/compound plates, a 96w and 384w pipette head, a heating and cooling module, a shaker, robot grippers and a flexible arm, shuttles to move the plates and barcode trackers. Attached to the liquid handling workstation are:

  • Agilent PlateLoc and Brooks XPeel – Thermal microplate sealer and automated microplate seal removal
  • Agilent Microplate Centrifuge – An automated small, robot-accessible centrifuge for multiple microplates
  • Cytomat 10C incubator – Fully automated incubator and storage module for high-capacity cell growth and assay incubation
  • Cytomat 9 hotel – Storage hotel for tip boxes and compound microplates
  • Echo 550 liquid handler – Acoustic liquid handling for precise nL transfer volumes of drug solutions
  • FluidX IntelliXcap Decapper – The fastest capper/decapper 96-format system in the industry, that can decap a complete rack of tubes in as little as 20 seconds
  • Thermo Multidrop Combi reagent dispenser – Provides precise dispensing of solutions, ensuring reproducible assay data
  • Spectramax i3x – Microplate reader for absorbance, luminescence and fluorescence detection
Personnel

Facility manager
Selma Eising

Assay development and application
Bianca Koopmans

Assay development and application
Kimberly Ober

Interested?

Contact: HTS@prinsesmaximacentrum.nl