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High-throughput screening (HTS)

Our aim is to facilitate the accelerated identification and validation of improved treatment strategies and disease-associated genes and proteins by supporting the development, performance and analysis of high-throughput perturbation assays. The HTS facility is embedded in the Molenaar group at the Princess Máxima Center for pediatric oncology.
What we offer:

The highly flexible HTS platform enables the performance of a variety of high-throughput screens, using different perturbations (e.g. small molecules, antibodies or siRNAs) and read-out methods (e.g. absorbance-, luminescence- or fluorescence-based detection or flow cytometric analysis).
Assay development and validation – we can give support in the translation of research questions into assays suitable for high-throughput screening.
Carrying out the high-throughput screens
Data analysis support
Provide compound libraries:

  •   The Prestwick Chemical Library (Prestwick), containing 1280 marketed drugs with high chemical and pharmacological diversity.
  • The Anti-Neoplastic KIT (Sequoia), containing 165 (recently) approved drugs used in cancer treatment.
  • The Spectrum Collection (MicroSource Discovery Systems), containing a selection of 2320 drug components, natural products and bioactive molecules.
  • The Screen-Well® Epigenetics Library (Enzo), containing 43 drugs with defined activity against epigenetics-modifying enzymes.
  • The Epigenetics Screening Library (Cayman), containing 54 compounds that modulate the activity of epigenetic enzymes and histone-binders.
  • The Cell Cycle/DNA Damage Compound Library (MedChemExpress), containing 451 cell cycle/DNA damage related compounds.
Our equipment:

The HTS facility consists of a liquid handling robot for the accurate and reproducible distribution and (serial) dilution of single solutions into 96- and 384-well plates. Plates can be transferred to the following integrated devices for incubation, further sample processing or read-out:

  • Automated CO2 incubator – for incubation of standard and deep well plates
  • Microplate reader – for absorbance, luminescence and fluorescence detection
  • HyperCyt® Rapid Sampler combined with the BD AccuriTM C6 Flow Cytometer
  • Vspin integrated microplate centrifuge (Agilent) – a centrifuge for standard and deep well microplates
  • Multidrop Combi reagent dispenser (Thermo Fisher Scientific) – an automated pipetting system for solution dispensing into 96- and 384-well plates
  • D300e Digital Dispenser (Tecan) – a benchtop dispenser for the rapid and accurate delivery of picoliter to microliter volumes of small molecules and biomolecules in DMSO or aqueous solution into 12-, 24-, 48-, 96-, 384- and 1536-well plates. The D300 Digital Dispenser is useful for the estimation of dose-response curves, for combination studies, for the setup of PCR reactions and for the generation of enzyme profiles
  • Plate sealer and peeler – suitable for polypropylene and polystyrene plates

The liquid handling robot additionally contains on-deck modules for shaking, heating, cooling and washing.

Personnel

Facility manager
Emmy Dolman

Assay development and application
Bianca Koopmans

Assay development and application
Anke Essing

Interested?

Contact Emmy Dolman
Email: M.E.M.Dolman-2@prinsesmaximacenterum.nl