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Seahorse Cell Metabolism Technology

Mitochondrial function and glycolysis are key in a variety of vital cellular processes, including cellular activation, proliferation, differentiation, cell death, and disease progression. As such, mitochondria play a crucial role in virtually all aspects of tumor progression. Embedded within the Hoeijmakers group, Seahorse Technology allows us to monitor changes in functional metabolism.
What we offer:
The Seahorse XFe24 Analyzer, can be used for measuring a variety of key metabolic parameters and functions in isolated mitochondria, living cells (adherent and suspension) and organoids, cultured human or mouse tissue slices and fresh patient’s material, all in a blinded randomized manner in a 24-well format. This includes oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), both as key indicators of mitochondrial respiration and glycolysis, and such measurements provide us a systems-level view of cellular metabolic function. It can be used to detect basal differences in oxidative phosphorylation and glycolysis of e.g. various cancer types, monitoring differential functional metabolism between healthy normal tissue and cancers as well as their responsiveness to therapy and interventions.

The ability to measure the metabolic phenotype of cells and the shift between respiration and glycolysis under pathological conditions will allow you to connect physiological traits of cells with genomic and proteomic data.

Assays possible include:
- Mito Stress test
- ATP-Rate assay
- Glycolytic Rate assay

Our equipment:
Seahorse XFe24 Analyzer (for now only at ambient oxygen)