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Flow Cytometry and Cell Sorting

Flow cytometry (FC) and cell sorting (CS) are essential technologies for a research institute focused on pediatric cancer. The ability to automatically characterize or sort individual cells based on characteristics such as the combination of surface markers presented on each, has been essential for research and diagnosis of leukemias for example. Coupling to other single cell techniques has greatly expanded the types of tumors and tissues for which FC and CS can be applied. The Princess Máxima FC and CS facility is an independent facility for the entire center. It is embedded within the Van Leeuwen group.
What we offer:

The Princess Máxima Center Flow Cytometry and Cell Sorting core facility aims at ensuring state-of-the-art equipment, maintaining and calibrating these, as well as providing world-class expertise and support for using this equipment for those research groups that require FC or CS. All equipment listed below is available for use by scientists at the Princess Máxima Center. The type of use and the cost charged is dependent on the degree to which a facility operator is required for use of the equipment. At the discretion of the facility operator, trained users are allowed to book and independently use some of the equipment. Training is also offered by the facility, specifically for users who make frequent and long-term use of the equipment. Before making use of any of the equipment it is essential to make an appointment with the facility operator, to discuss the requirements of your project, cost estimates, instructions for booking and use, as well as an explanation of the rules of the facility. Besides offering state-of-the-art facilities, the facility is run in close collaboration with diagnostics to ensure seamless transition of important discoveries to clinical care. In addition, we are in close contact with the other cytometry facilities on campus to ensure exchange of knowledge and capacity.

Our equipment:

Room 3.09b (MLI)

  • AstriosEQ            5 laser, 23 colour cell sorter (can be used for MLII work if required)
  • Cytoflex LX         6 laser, 21 colour analyser                                                        
  • Biorad ZE5          5 laser, 27 colour analyser                                                                                                                                

Room 3.09a (MLII)

  • SONY SH800S   4 laser, 6 colour cell sorter (also for use independent of operator)
  • Cytoflex S           4 laser, 13 colour analyser                                                        
  • MacsQuant         3 laser, 8 colour

Contact Tomasz Poplonski
Email: T.M.Poplonski-2@prinsesmaximacentrum.nl