Purpose for research
At the Princess Máxima Center Protein Facility, we have broad expertise in protein/antibody biochemistry and engineering, including:
- The expression, purification, and characterization of recombinant proteins (cytoplasmic, secreted, and membrane proteins) and antibodies.
- In vitro display technologies (phage display, yeast display, and ribosome display) for antibody discovery and protein engineering.
- Structure-function analysis of ligand-receptor interactions.
We collaborate closely with research groups inside and outside of the Máxima and other core facilities in different projects to leverage proteins and antibodies for innovative approaches to diagnose and treat childhood cancer.
Currently, we have ongoing projects on:
- Developing nanobody-based radiotracers for non-invasive PET imaging and radioimmunotherapy (in collaboration with dr. Max van Noesel and dr. Alex Poot of the Theranostics Facility at UMC Utrecht).
- Developing chimeric antigen receptors for CAR T-cell therapy (in collaboration with the CAR T- Cell Program, the Cell Therapy Facility, and the Single Cell Genomics Facility of the Princess Máxima Center).
- Engineering ligands with novel signaling properties for enhancing organoid growth and modulating signaling pathways of interest for regenerative medicine.
- Preparing recombinant proteins and enzymes for in vitro and in vivo functional studies.